期刊
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
卷 17, 期 35, 页码 23245-23256出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cp03329b
关键词
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资金
- National Science Foundation (CAREER Award) [CBET-0952624, CBET-1510099]
- Alzheimer Association - New Investigator Research Grant [2015-NIRG-341372]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [0952624, 1510099] Funding Source: National Science Foundation
Epidemiological studies have shown that the development of Alzheimer's disease (AD) is associated with type 2 diabetes (T2D), but it still remains unclear how AD and T2D are connected. Heterologous cross-seeding between the causative peptides of A beta and hIAPP may represent a molecular link between AD and T2D. Here, we computationally modeled and simulated a series of cross-seeding double-layer assemblies formed by A beta and hIAPP peptides using all-atom and coarse-gained molecular dynamics (MD) simulations. The cross-seeding A beta-hIAPP assemblies showed a wide range of polymorphic structures via a combination of four beta-sheet-to-beta-sheet interfaces and two packing orientations, focusing on a comparison of different matches of beta-sheet layers. Two cross-seeding A beta-hIAPP assemblies with different interfacial beta-sheet packings exhibited high structural stability and favorable interfacial interactions in both oligomeric and fibrillar states. Both A beta-hIAPP assemblies displayed interfacial dehydration to different extents, which in turn promoted A beta-hIAPP association depending on interfacial polarity and geometry. Furthermore, computational mutagenesis studies revealed that disruption of interfacial salt bridges largely disfavor the beta-sheet-to-beta-sheet association, highlighting the importance of salt bridges in the formation of cross-seeding assemblies. This work provides atomic-level information on the cross-seeding interactions between A beta and hIAPP, which may be involved in the interplay between these two disorders.
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