期刊
JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH
卷 40, 期 3, 页码 770-778出版社
WILEY
DOI: 10.1111/jog.12252
关键词
endometriosis; inflammation; interleukin-8; resveratrol; sirtinol; tumor necrosis factor-
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Grants-in-Aid for Scientific Research [23592395, 24592505] Funding Source: KAKEN
AimEndometriosis is a chronic inflammatory disease. Sirtuin 1 (SIRT1) plays a role in regulation of inflammation. The role of SIRT1 in endometriosis remains unknown. We here addressed the anti-inflammatory effects of SIRT1 on endometriosis. MethodsThe expression of SIRT1 in human ovarian endometriomas and eutopic endometria were examined using immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). Endometriotic stromal cells (ESC) obtained from endometriomas were exposed to either resveratrol or sirtinol, an activator or inhibitor of sirtuins, respectively, and tumor necrosis factor (TNF)--induced interleukin (IL)-8 release from the ESC was assessed at mRNA and protein levels. ResultsBoth immunochemistry and RT-PCR demonstrated that SIRT1 was expressed in ESC and normal endometrial stromal cells. Resveratrol suppressed TNF--induced IL-8 release from the ESC in a dose-dependent manner while sirtinol increased IL-8 release. ConclusionThese opposing effects of SIRT1-related agents suggest that IL-8 release from the ESC is modulated through the SIRT1 pathway. Resveratrol may have the potential to ameliorate local inflammation in endometriomas.
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