期刊
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 24, 期 7, 页码 1401-1413出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2012.11.008
关键词
Polyphenol extract; Extra virgin olive oil; Ulcerative colitis; PPAR gamma; I kappa B alpha
资金
- Ministerio de Ciencia y Tecnologia [AGL2008-02475]
- Junta de Andalucia, Spain
We evaluated the protective effect of dietary extra virgin olive oil (EVOO) polyphenol extract (PE) supplementation in the inflammatory response associated to chronic colitis model. Six-week-old mice were randomized in four dietary groups: standard diet (SD), EVOO diet and both enriched with PE (850 ppm) (SD +PE and EVOO+PE). After 30 days, animals that were exposed to dextran sodium sulfate (DSS) (3%) followed by 3 weeks of drinking water developed chronic colitis, which was evaluated by disease activity index (DAI) and histology. Cell proliferation was analyzed by immunohistochemical and changes in monocyte chemotactic protein (MCP)-1 and tumor necrosis factor (TNF)-alpha mRNA expression by quantitative real-time polymerase chain reaction. Colonic expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, mitogen-activated protein kinases (MAPKs), I kappa B alpha inhibitory and peroxisome proliferator-activated receptor gamma (PPAR gamma) were determined by western blotting. SD-DSS group showed a significant increase of DAI, histological damage and cell proliferation, as well as an up-regulation of TNF-alpha, MCP-1, COX-2 and iNOS proteins. p38 and INK MAPKs phosphorylation, I kappa B alpha degradation and PPAR gamma deactivation were also observed. However, in DSS-treated and EVOO+PE-fed mice, DAI and cell proliferation were significantly reduced, as well as MCP-1, TNF-alpha, COX-2 and iNOS expression levels. In addition, this dietary group, notably down-regulated JNK phosphorylation, prevented I kappa B alpha degradation and PPAR gamma deactivation. These results demonstrated, for the first time, that EVOO-PE supplementation possessed marked protective effects on experimental colitis through PPAR gamma up-regulation and nuclear transcription factor-kappa B and MAPK signaling pathway inhibition, decreasing the inflammatory cascade. We concluded that PE-enriched EVOO diet could be a beneficial functional food on ulcerative colitis. (C) 2013 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据