期刊
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 20, 期 11, 页码 860-865出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2008.07.014
关键词
Hepcidin; H9C2 cardiomyocytes; Ferroportin 1; Hephaestin; Iron release; Heart iron
资金
- The Hong Kong Research Grants Council [CUHK466907-KY]
- Chinese University of Hong Kong [4450226-KY]
- Jiangsu Natural Science Foundation [05-BK2005430]
- Hong Kong Polytechnic University [GU-384, G-YG11]
- National Key Laboratory of Chinese Medicine and Molecular Pharmacology (Shenzhen)
- Shenzhen-Hong Kong Innovation Circle Program
Iron plays a key pathophysiological role in a number of cardiac diseases. Studies on the mechanisms of heart iron homeostasis are therefore crucial for understanding the causes of excessive heart iron. In addition to iron uptake, cellular iron balance in the heart also depends on iron export. We provided evidence for the existence of iron exporter ferroportin 1 (Fpn 1) in the heart in a recent study. The presence of hepcidin, a recently discovered iron regulatory hormone, was also confirmed in the heart recently. Based on these findings and the inhibiting role of hepcidin on Fpn I in other tissues, we speculated that hepcidin might be able to bind with, internalize and degrade Fpn I and then decrease iron export in heart cells, leading to an abnormal increase in heart iron and iron mediated cell injury. We therefore investigated the effects of hepcidin on the contents of Fpn I and iron release in H9C2 cardiomyocyte cell line. We demonstrated that hepcidin has the ability to reduce Fpn I content as well as iron release in this cell. The similar regulation patterns of hepcidin on the Fpn I and iron release suggested that the decreased iron release resulted from the decreased content of Fpn I induced by hepcidin. We also found that hepcidin has no significant effects on ceruloplasmin (CP) and hephaestin (Heph) - two proteins required for iron release from mammalian cells. The data imply that Fpn1, rather than Heph and CP, is the limited factor in the regulation of iron release from heart cells under physiological conditions. (C) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据