期刊
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
卷 19, 期 4, 页码 237-245出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jnutbio.2007.02.013
关键词
lipogenesis; gene expression; VLDL; triacylglycerol; palmitic acid; MIDA
资金
- NATIONAL CENTER FOR RESEARCH RESOURCES [M01RR000102] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK040995, P30DK026687] Funding Source: NIH RePORTER
- NCRR NIH HHS [M01 RR000102-390372, M01 RR000102, M01 RR 00102] Funding Source: Medline
- NIDDK NIH HHS [P30 DK026687, P30 DK026687-27, P30 DK 26687, DK 40995] Funding Source: Medline
Background: Hepatic de novo lipogenesis (DNL) is markedly stimulated in humans by low-fat diets enriched in simple sugars. However, the dietary responsiveness of the key enzyme controlling DNL in human adipose tissue, fatty acid synthase (FAS), is uncertain. Hypothesis: Adipose tissue mRNA for FAS is increased in lean and obese subjects when hepatic DNL is elevated by a eucaloric, low-fat, high-sugar diet. Design: Twelve lean and seven obese volunteers were given two eucaloric diets (10% vs. 30% fat; 75% vs. 55% carbohydrate; sugar/starch 60/40) each for 2 weeks by a random-order cross-over design. FAS mRNA in abdominal and gluteal adipose tissues was compared to hepatic DNL measured in serum by isotopic and nonisotopic methods. Adipose tissue mRNA for tumor necrosis factor-alpha and IL-6, which are inflammatory cytokines that modulate DNL, was also assayed. Results: The low-fat high-sugar diet induced a 4-fold increase in maximum hepatic DNL (P<001) but only a 1.3-fold increase in adipose tissue FAS mRNA (P=.029) and no change in cytokine mRNA. There was a borderline significant positive correlation between changes in FAS mRNA and hepatic DNL (P=.039). Compared to lean subjects, obese subjects had lower levels of FAS mRNA and higher levels of cytokine mRNA (P<001). Conclusions: The results suggest that key elements of human adipose tissue DNL are less responsive to dietary carbohydrate than is hepatic DNL and may be regulated by diet-independent factors. Irrespective of diet, there is reduced expression of the FAS gene and increased expression of cytokine genes in adipose tissues of obese subjects. (C) 2008 Elsevier Inc. All rights reserved.
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