期刊
JOURNAL OF NUTRITION
卷 145, 期 1, 页码 25-31出版社
OXFORD UNIV PRESS
DOI: 10.3945/jn.114.202515
关键词
glutamine; tight junction; corticotropin-releasing factor; pigs; small intestine; weaning stress
资金
- National Basic Research Program of China [2013CB127302]
- National Natural Science Foundation of China [31172217, 31272450, 31272451]
- Chinese Universities Scientific Fund [2013RC002]
- Program for New Century Excellent Talents in University [NCET-12-0522]
- Program for Beijing Municipal Excellent Talents
- Agriculture and Food Research Initiative competitive grant from USDA National Institute of Food and Agriculture [2014-67015-21770]
- Texas A&M AgriLife Research [H-8200]
Background: Dysfunction of tight junction integrity is associated with decreased nutrient absorption and numerous gastrointestinal diseases in humans and piglets. Although L-glutamine has been reported to enhance intestinal-mucosal mass and barrier function under stressful conditions, in vivo data to support a functional role for L-glutamine on intestinal tight junction protein (TJP) expression in weanling mammals are limited. Objective: This study tested the hypothesis that glutamine regulates expression of TJPs and stress-related corticotropin-releasing factor (CRF) signaling in the jejunum of weanling piglets. Methods: Piglets were reared by sows or weaned at 21 d of age to a corn and soybean meal based diet that was or was not supplemented with 1% L-glutamine for 7 d. Growth performance, intestinal permeability, TJP abundance, and CRF expression were examined. Results: Weaning caused increases (P < 0.05) in intestinal permeability by 40% and in ORE concentrations by 4.7 times in association with villus atrophy (P < 0.05). Western blot analysis showed reductions (P < 0.05) in jejunal expression of occludin, claudin-1, zonula occludens (ZO) 2, and ZO-3, but no changes in the abundance of claudin-3, claudin-4, or ZO-1 in weanling piglets compared with age-matched suckling controls. Glutamine supplementation improved (P < 0.05) intestinal permeability and villus height, while reducing (P < 0.05) jejunal mRNA and protein levels for CRF and attenuating (P < 0.05) weanling-induced decreases in occludin, claudin-1, ZO-2, and ZO-3 protein abundances. Conclusion: Collectively, our results support an important role for L-glutamine in regulating expression of TJPs and CRF in the jejunum of weanling piglets.
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