期刊
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
卷 17, 期 11, 页码 7548-7554出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cp00439j
关键词
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资金
- Marie Curie individual Fellowship [MEIT-CT-2006-041522]
- EPSRC
- Engineering and Physical Sciences Research Council [1231179, EP/I003983/1, EP/K030760/1] Funding Source: researchfish
- EPSRC [EP/K030760/1, EP/I003983/1] Funding Source: UKRI
Microviscosity is of paramount importance in materials and bio-sciences. Fluorescence imaging using molecular rotors has emerged as a versatile tool to measure microviscosity, either using a fluorescence lifetime or a ratiometric signal of the rotor; however, only a limited number of blue-to-green-emitting fluorophores with both the lifetime and the ratiometric signal sensitivity to viscosity have been reported to date. Here we report a deep red emitting dual viscosity sensor, which allows both the ratiometric and the lifetime imaging of viscosity. We study viscosity in a range of lipid-based systems and conclude that in complex dynamic systems dual detection is preferable in order to independently verify the results of the measurements as well as perform rapid detection of changing viscosity.
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