期刊
JOURNAL OF NUTRITION
卷 142, 期 12, 页码 2105-2111出版社
AMER SOC NUTRITION-ASN
DOI: 10.3945/jn.112.166462
关键词
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资金
- NIH [R01 DK 081551]
- USDA Regional Research [W-2002]
- California Agricultural Experiment Station
- National Center for Research Resources, a component of the NIH [UL1 RR024146]
- NIH Roadmap for Medical Research
- U.S. Department of Energy by Lawrence Livermore National Laboratory [DE-AC52-07NA27344]
- NIH National Center for Research Resources [RR13461]
Kinetic models enable nutrient needs and kinetic behaviors to be quantified and provide mechanistic insights into metabolism. Therefore, we modeled and quantified the kinetics, bioavailability, and metabolism of RRR-alpha-tocopherol in 12 healthy adults. Six men and 6 women, aged 27 +/- 6 y, each ingested 1.81 nmol of [5(-14)CH(3)]-(2R, 4'R, 8'R)-alpha-tocopherol; each dose had 3.70 kBq of C-14. Complete collections of urine and feces were made over the first 21 d from dosing. Serial blood samples were drawn over the first 70 d from dosing. All specimens were analyzed for RRR-alpha-tocopherol. Specimens were also analyzed for C-14 using accelerator MS. From these data, we modeled and quantified the kinetics of RRR-alpha-tocopherol in vivo in humans. The model had 11 compartments, 3 delay compartments, and reservoirs for urine and feces. Bioavailability of RRR-alpha-tocopherol was 81 +/- 1%. The model estimated residence time and half-life of the slowest turning-over compartment of alpha-tocopherol (adipose tissue) at 499 +/- 702 d and 184 +/- 48 d, respectively. The total body store of RRR-alpha-tocopherol was 25,900 +/- 6=220 mu mol (11 +/- 3 g) and we calculated the adipose tissue level to be 1.53 mu mol/g (657 mu g/g). We found that a daily intake of 9.2 mu mol (4 mg) of RRR-alpha-tocopherol maintained plasma RRR-alpha-tocopherol concentrations at 23 mu mol/L. These findings suggest that the dietary requirement for vitamin E may be less than that currently recommended and these results will be important for future updates of intake recommendations. J. Nutr. 142: 2105-2111, 2012.
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