期刊
JOURNAL OF NUCLEAR MEDICINE
卷 55, 期 10, 页码 1699-1705出版社
SOC NUCLEAR MEDICINE INC
DOI: 10.2967/jnumed.114.139428
关键词
oxidized LDL; macrophage; F-18-FDG; PET; reactive oxygen species; HIF-1 alpha
资金
- Samsung Biomedical Research Institute [SMX1131891]
- New Faculty Research Fund at the Ajou University School of Medicine
For F-18-FDG PET to be widely used to monitor atherosclerosis progression and therapeutic response, it is crucial to better understand how macrophage glucose metabolism is influenced by the atherosclerotic microenvironment and to elucidate the molecular mechanisms of this response. Oxidized low-density lipoprotein (oxLDL)is a key player in atherosclerotic inflammation that promotes macrophage recruitment, activation, and foam cell formation. We thus explored the effect of oxLDL on macrophage F-18-FDG uptake and investigated the underlying molecular mechanism including the roles of hypoxia-inducible factor-1 alpha (HIF-1 alpha) and reactive oxygen species (ROS). Methods: RAW264.7 macrophages were stimulated with native LDL, oxLDL, or lipopolysaccharide. Cells were assessed for F-18-FDG uptake, lactate production, membrane glucose transporter 1 (GLUT1) expression, and hexokinase activity. ROS generation, Nox expression, and HIF-1 alpha activity were also measured. Results: oxLDL (20 mu g/mL) induced a 17.5 +/- 1.7-fold increase in macrophage F-18-FDG uptake by 24 h, which was accompanied by increased lactate production, membrane GLUT1 expression, and hexokinase activity. oxLDL-stimulated F-18-FDG uptake was completely blocked by inhibitors of Src or phosphoinositide 3-kinase. ROS generation was increased to 262.4% +/- 17.9% of controls by oxLDL, and N-acetyl-L-cysteine completely abrogated both oxLDL-induced ROS production and F-18-FDG uptake. oxLDL increased Nox2 expression, and nicotinamide adenine dinucleotide phosphate oxidase inhibition totally blocked increased ROS generation and F-18-FDG uptake by oxLDL. Finally, there was a clear ROS-dependent increase of HIF-1 alpha accumulation by oxLDL, and silencing of HIF-1 alpha completely abolished the metabolic effect of oxLDL. Conclusion: oxLDL is a strong stimulator of macrophage F-18-FDG uptake and glycolysis through upregulation of GLUT1 and hexokinase. This metabolic response is mediated by Nox2-dependent ROS generation that promotes HIF-1 alpha activation.
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