期刊
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
卷 17, 期 32, 页码 20882-20888出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5cp03377b
关键词
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资金
- JSPS [26800222]
- MEXT of Japan [15H00806, 26289311]
- Grants-in-Aid for Scientific Research [15H05928, 26289311, 26800222, 15H00806] Funding Source: KAKEN
Macro-phase separation in mixed lipid membranes containing the hybrid lipid palmitoyloleoylphosphatidylcholine (POPC) was observed by fluorescent and confocal laser scanning microscopy. In a binary system consisting of the saturated lipid dipalmitoylphosphatidylcholine (DPPC) and the hybrid lipid POPC, the hybrid lipid forms a liquid-disordered (L-d) phase. In a ternary system consisting of this binary system and an unsaturated lipid dioleoylphosphatidylcholine (DOPC), three-phase coexistence is observed. The POPC-rich phase appears around DPPC-rich domains, and the hybrid lipid is expected to behave like a line-active agent (linactant). Finally, phase separation in a four-component system, composed of this ternary system and cholesterol, was examined. Domains with a size that is smaller than 1 mm are found, and domain-induced budding is also observed. To explain small domain formation and domain-induced budding, chain ordering was evaluated based on Laurdan generalized polarization measurements. Our observations revealed that the hybrid lipid acted like a linactant to solid domains and disturbed chain ordering in liquid-ordered (L-o) domains. In both cases, the hybrid lipid reduced line tension at the domain boundary.
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