期刊
JOURNAL OF NEUROSCIENCE RESEARCH
卷 91, 期 3, 页码 444-452出版社
WILEY-BLACKWELL
DOI: 10.1002/jnr.23173
关键词
Dichlorvos; microglia; cell cycle; p53; p21; ATM
资金
- Council of Scientific and Industrial Research (CSIR), New Delhi, India
- Indian Council of Medical Research (ICMR)
Dichlorvos, an organophosphate (OP), is known to cause oxidative stress in the central nervous system (CNS). Previously we have shown that dichlorvos treatment promoted the levels of proinflammatory molecules and ultimately induced apoptotic cell death in primary microglial cells. Here we studied the effect of dichlorvos on crucial cell cycle regulatory proteins and the DNA damage sensor ataxia-telangiectasia mutated (ATM). We found a significant increase in p53 and its downstream target, p21, levels in dichlorvos-treated microglial cells compared with control cells. Moreover, dichlorvos exposure promoted the levels of different cell cycle regulatory proteins. These results along with flow cytometry results suggested that primary microglial cells were arrested at G1 and G2/M phase after dichlorvos exposure. We have shown in a previous study that dichlorvos can induce DNA damage in microglia; here we found that microglial cells also tried to repair this damage by inducing a DNA repair enzyme, i.e., ATM. We observed a significant increase in the levels of ATM after dichlorvos treatment compared with control. (c) 2012 Wiley Periodicals, Inc.
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