期刊
JOURNAL OF NEUROSCIENCE RESEARCH
卷 89, 期 9, 页码 1489-1498出版社
WILEY
DOI: 10.1002/jnr.22678
关键词
HAM/TSP; MT-2; SH-SY5Y; CDK5-siRNA; Tax
资金
- FONDECYT [108-0396]
Human T-cell leukemia virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a neurodegenerative disease characterized by selective loss of axons and myelin in the corticospinal tracts. This central axonopathy may originate from the impairment of anterograde axoplasmic transport. Previous work showed tau hyperphosphorylation at T-181 in cerebrospinal fluid of HAM/TSP patients. Similar hyperphosphorylation occurs in SH-SY5Y cells incubated with supernatant from MT-2 cells (HTLV-I-infected lymphocytes secreting viral proteins, including Tax) that produce neurite shortening. Tau phosphorylation at T 181 is attributable to glycogen synthase kinase 3-beta (GSK3-beta) and cyclin-dependent kinase 5 (CDK5) activation. Here we investigate whether neurite retraction in the SH-SY5Y model associates with concurrent changes in other tau hyperphosphorylable residues. Threonine 181 turned out to be the only tau hyperphosphorylated residue. We also evaluate the role of GSK3-beta and CDK5 in this process by using specific kinase inhibitors (LiCl, TDZD-8, and roscovitine). Changes in both GSK3-beta active and inactive forms were followed by measuring the regulatory phosphorylable sites (S-9 and Y-216, inactivating and activating phosphorylation, respectively) together with changes in beta-catenin protein levels. Our results showed that LiCl and TDZD-8 were unable to prevent MT-2 supernatant-mediated neurite retraction and also that neither Y-216 nor S-9 phosphorylations were changed in GSK3-beta. Thus, GSK3-beta seems not to play a role in T 181 hyperphosphorylation. On the other hand, the CDK5 involvement in tau phosphorylation was confirmed by both the increase in its enzymatic activity and the absence of MT-2 neurite retraction in the presence of roscovitine or CDK5 siRNA transfection. (C) 2011 Wiley-Liss, Inc.
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