期刊
JOURNAL OF NEUROSCIENCE RESEARCH
卷 88, 期 7, 页码 1394-1405出版社
WILEY
DOI: 10.1002/jnr.22322
关键词
spinal cord injury; galectin-1; common marmoset; neural stem/progenitor cells; preclinical study
资金
- Japanese Ministry of Education, Sports and Culture
- Human Frontier Science Program Organization
- Core Research for Evolutional Science and Technology (CREST) from the Japan Science and Technology Corporation (JST)
- General Insurance Association of Japan
- High-Tech Research Center in a Private University in Japan
- Keio University
- Ministry of Education, Culture, Sports, Science and Technology, Japan
Delayed transplantation of neural stem/progenitor cells (NS/PCs) into the injured spinal cord can promote functional recovery in adult rats and monkeys. To enhance the functional recovery after NS/PC transplantation, we focused on galectin-1, a carbohydrate-binding protein with pleiotropic roles in cell growth, differentiation, apoptosis, and neurite outgrowth. Here, to determine the combined therapeutic effect of NS/PC transplantation and galectin-1 on spinal cord injury (SCI), human NS/PCs were transfected by lentivirus with galectin-1 and green fluorescent protein (GFP), (Gal-NS/PCs) or GFP alone (GFP-NS/PCs), expanded in vitro, and then transplanted into the spinal cord of adult common marmosets, 9 days after contusive cervical SCI. The animals' motor function was evaluated by their spontaneous motor activity, bar grip power, and performance on a treadmill test. Histological analyses revealed that the grafted human NS/PCs survived and differentiated into neurons, astrocytes, and oligodendrocytes. There were significant differences in the myelinated area, corticospinal fibers, and serotonergic fibers among the Gal-NS/PC, GFP-NS/PC, vehicle-control, and sham-operated groups. The Gal-NS/PC-grafted animals showed a better performance on all the behavioral tests compared with the other groups. These findings suggest that Gal-NS/PCs have better therapeutic potential than NS/PCs for SCI in nonhuman primates and that human Gal-NS/PC transplantation might be a feasible treatment for human SCI. (C) 2010 Wiley-Liss, Inc.
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