期刊
JOURNAL OF NEUROSCIENCE RESEARCH
卷 88, 期 13, 页码 2833-2846出版社
WILEY
DOI: 10.1002/jnr.22440
关键词
rat; red nucleus; olfactory ensheathing cells; cell body treatment; systemic treatment cAMP; dbcAMP; regeneration; spinal cord injury
资金
- Fond de Recherche en Sante du Quebec (FRSQ)
- Canadian Institutes of Health Research (CHIR)
- Michael Smith Foundation for Health Research
- Christopher and Dana Reeve Foundation
- International Spinal Research Trust
The failure of CNS axons to regenerate following traumatic injury is due in part to a growth-inhibitory environment in CNS as well as a weak intrinsic neuronal growth response. Olfactory ensheathing cell (OECs) transplants have been reported to create a favorable environment promoting axonal regeneration, remyelination, and functional recovery after spinal cord injury. However, in our previous experiments, OEC transplants failed to promote regeneration of rubrospinal axons through and beyond the site of a dorsolateral funiculus crush in rats. Rubrospinal neurons undergo massive cell atrophy and limited expression of regeneration-associated genes after axotomy. Using the same injury model, we tested the hypothesis that treatment of the red nucleus with cAMP, known to stimulate the intrinsic growth response in other neurons, will promote rubrospinal regeneration in combination with OEC transplants. In addition, we assessed a systemic increase of cAMP using the phosphodiesterase inhibitor rolipram. OECs prevented cavity formation, attenuated astrocytic hypertrophy and the retraction of the axotomized rubrospinal axons, and tended to reduce the overall lesion size. OEC transplantation lowered the thresholds for thermal sensitivity of both forepaws. None of our treatments, alone or in combination, promoted rubrospinal regeneration through the lesion site. However, the systemic elevation of cAMP with rolipram resulted in greater numbers of OECs and axonal density within the graft and improved motor performance in a cylinder test in conjunction with enhanced rubrospinal branching and attenuated astrocytic hypertrophy. (C) 2010 Wiley-Liss, Inc.
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