期刊
JOURNAL OF NEUROSCIENCE RESEARCH
卷 88, 期 10, 页码 2217-2227出版社
WILEY
DOI: 10.1002/jnr.22372
关键词
whole-cell patch clamp; PrP(106-126); potassium currents; diagonal band of Broca
资金
- Alberta Prion Research Institute
- Alberta Ingenuity Fund
- Canada Research Chairs Program
- Canadian Institutes of Health Research [MOP36377]
Prion diseases are neurodegenerative disorders that are characterized by the presence of the misfolded prion protein (PrP). Neurotoxicity in these diseases may result from prion-induced modulation of ion channel function, changes in neuronal excitability, and consequent disruption of cellular homeostasis. We therefore examined PrP effects on a suite of potassium (K+) conductances that govern excitability of basal forebrain neurons. Our study examined the effects of a PrP fragment [PrP(106-126), 50 nM] on rat neurons using the patch clamp technique. In this paradigm, PrP(106-126) peptide, but not the scrambled sequence of PrP(106-126), evoked a reduction of whole-cell outward currents in a voltage range between -30 and +30 mV. Reduction of whole-cell outward currents was significantly attenuated in Ca2+-free external media and also in the presence of iberiotoxin, a blocker of calcium-activated potassium conductance. PrP(106-126) application also evoked a depression of the delayed rectifier (I-K) and transient outward (I-A) potassium currents. By using single cell RTPCR, we identified the presence of two neuronal chemical phenotypes, GABAergic and cholinergic, in cells from which we recorded. Furthermore, cholinergic and GABAergic neurons were shown to express K(v)4.2 channels. Our data establish that the central region of PrP, defined by the PrP(106-126) peptide used at nanomolar concentrations, induces a reduction of specific K+ channel conductances in basal forebrain neurons. These findings suggest novel links between PrP signalling partners inferred from genetic experiments, K+ channels, and PrP-mediated neurotoxicity. (C) 2010 Wiley-Liss, Inc.
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