期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 222, 期 -, 页码 82-90出版社
ELSEVIER
DOI: 10.1016/j.jneumeth.2013.10.014
关键词
Micropattern; Axon tract; Inhibitory afferent; Electrical stimulation; Calcium imaging; Interface
资金
- NIH (P41 Tissue Engineering Resource Center) [EB002520, EY020856]
- Tufts Center for Neuroscience Research Core Award
The cortical circuitry in the brain consists of structurally and functionally distinct neuronal assemblies with reciprocal axon connections. To generate cell culture-based systems that emulate axon tract systems of an in vivo neural network, we developed a living neural circuit consisting of compartmentalized neuronal populations connected by arrays of two millimeter-long axon tracts that are integrated on a planar multi-electrode array (MEA). The millimeter-scale node-to-node separation allows for pharmacological and electrophysiological manipulations to simultaneously target multiple neuronal populations. The results show controlled selectivity of dye absorption by neurons in different compartments. MEA-transmitted electrical stimulation of targeted neurons shows similar to 46% increase of intracellular calcium levels with 20 Hz stimulation, but similar to 22% decrease with 2k Hz stimulation. The unique feature of long distance axons promotes in vivo-like fasciculation. These axon tracts are determined to be inhibitory afferents by showing increased action potential firing of downstream node upon selective application of gamma-aminobutyric acid (GABA) to the upstream node. Together, this model demonstrates integrated capabilities for assessing multiple endpoints including axon tract tracing, calcium influx, network architecture and activities. This system can be used as a multi-functional platform for studying axon tract-associated CNS disorders in vitro, such as diffuse axonal injury after brain trauma. (C) 2013 Elsevier B.V. All rights reserved.
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