期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 218, 期 2, 页码 206-213出版社
ELSEVIER
DOI: 10.1016/j.jneumeth.2013.03.004
关键词
Histology; Tissue freezing and sectioning; 3-D registration and reconstruction; High-throughput processing
资金
- National Institutes of Health [RC1MH088659]
- Office of the NIH Director [R01MH087988]
- Crick-Clay Professorship
- Cold Spring Harbor Laboratory
A major challenge in the histological sectioning of brain tissue is achieving accurate alignment in the standard coronal, horizontal, or sagittal planes. Correct alignment is desirable for ease of subsequent analysis and is a prerequisite for computational registration and algorithm-based quantification of experimental data. We have developed a simple and low-cost technique for whole-brain cryosectioning of rodent brains that reliably results in a precise alignment of stereotactic coordinates. The system utilises a 3-D printed model of a mouse brain to create a tailored cavity that is used to align and support the brain during freezing. The alignment of the frozen block is achieved in relation to the fixed edge of the mold. The system also allows for two brains to be frozen and sectioned simultaneously. System components, procedural steps, and examples of the end results are presented. (c) 2013 Elsevier B.V. All rights reserved.
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