期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 218, 期 1, 页码 103-109出版社
ELSEVIER
DOI: 10.1016/j.jneumeth.2013.05.004
关键词
Golgi-Cox staining; Electron microscopy; Ultrahigh-voltage electron microscopy; Golgi-EM method
资金
- Nanotechnology Platform of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan [12024046]
The introduction of the FD Rapid GolgiStain Kit has enabled many researchers to easily examine the morphology of neurons. Owing to an improved Golgi-Cox impregnation method, both stable and highly sensitive stained images of neurons can be acquired using this kit. To analyse microstructures such as dendritic spines and growth cones at detail, we attempted to create a useful method combining Golgi-Cox staining and electron microscopy (Golgi-EM method) so that the prepared Kit-stained samples would be appropriate for use in electron microscopy (EM) studies. Results from the present study suggest the importance of the glutaraldehyde fixation step in the Golgi-EM method. Here, we introduce the recommended protocol for the Golgi-EM method, which is available for ultrahigh-voltage electron microscopy and transmission electron microscopy. We are confident that our Golgi-EM method may become a reliable and important method that can contribute to new discoveries in neuronal morphology. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据