期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 198, 期 1, 页码 99-102出版社
ELSEVIER
DOI: 10.1016/j.jneumeth.2011.03.003
关键词
1,4-Benzoquinone; Immunofluorescence; CD 133; Glioblastoma stem cells
资金
- NCI NIH HHS [R01 CA090851-09, R01 CA090851] Funding Source: Medline
Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1.4-benzoquinone) for immunoflourescence staining of neurospheres. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据