期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 179, 期 2, 页码 166-172出版社
ELSEVIER
DOI: 10.1016/j.jneumeth.2009.01.019
关键词
Retrograde; Calcium; Imaging; Electrical stimulation; Retinal ganglion cells; Retina; Dextran
资金
- National Science Foundation [EEC-0310723]
- WM Keck Foundation
- Albaugh trust
Here we present a retrograde loading technique that makes it possible for the first time to rapidly load a calcium indicator in the majority of retinal ganglion cells (RGCs) in salamander retina, and then to observe physiological activity of these dye-loaded cells. Dextran-conjugated calcium indicator, dissolved in water. was applied to the optic nerve stump. Following dye loading, the isolated retina was mounted on a microelectrode array to demonstrate that electrical activity and calcium activity were preserved, as the retina responded to electrical stimuli. (C) 2009 Elsevier B.V. All rights reserved.
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