期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 173, 期 2, 页码 286-290出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2008.06.013
关键词
endothelin-1; focal ischemia; mouse; receptor; endothelial nitric oxide
资金
- Intramural NIH HHS [Z01 NS002792] Funding Source: Medline
- NINDS NIH HHS [R01 NS2792, R01 NS027292, P01 NS37520, R01 NS027292-16, P01 NS037520, P01 NS037520-10] Funding Source: Medline
intracerebral injection of the vasoconstrictor peptide, endothelin-1 (ET-1), has been used as a method to induce focal ischemia in rats. The relative technical simplicity of this model makes it attractive for use in mice. However, the effect of ETA on mouse brains has not been firmly established. In this study, we determined the ability of ET-1 to induce focal cerebral ischemia in four different mouse strains (CD1, C57/BL6, NOD/SCID, and FVB). In contrast to rats, intracerebral injection of ET-1 did not produce a lesion in any mouse strain tested. A combination of ET-1 injection with either CCA occlusion or N(G)-nitro-L-arginine methyl ester (L-NAME) injection produced only a small infarct and its size was strain-dependent. A triple combination of CCA occlusion with co-injection of ETA and L-NAME produced a lesion in all mouse strains tested, and this resulted in a significant motor deficit. However, lesion size was still relatively small and strain-dependent. This study shows that ET-1 has a much less potent effect for producing an infarct in mice than rats. (c) 2008 Elsevier B.V. All rights reserved.
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