期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 169, 期 1, 页码 214-221出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2007.11.029
关键词
retinal ganglion cell; in vivo imaging; fluorescence imaging
资金
- NEI NIH HHS [R01 EY002120, R56 EY002120, P30 EY001765-229001, P30 EY001765, EY 02120, EY01765] Funding Source: Medline
We have developed a technique that permits time-lapse imaging of retinal ganglion cells (RGCs), their dendritic arbors and their axons in mammals in vivo. This technique utilizes a standard confocal laser scanning microscope, transgenic mice that express yellow fluorescent protein (YFP) in a subset of RGCs and survival anesthesia techniques. The same individual RGCs with their dendritic arbors and axons were multiply imaged in vivo in both adult and juvenile mice. Additionally, the same RGC that was imaged in vivo could then be located and imaged in fixed retinal whole mount preparations. This novel technique has many potential applications. (c) 2007 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据