期刊
JOURNAL OF NEUROSCIENCE METHODS
卷 167, 期 2, 页码 292-301出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneumeth.2007.08.028
关键词
neuronal culture; physiological glucose; AMP-activated protein kinase; ATP; AICAR; C75
资金
- NINDS NIH HHS [R01 NS050625-01, R01 NS050625, R01 NS050625-03, R01 NS050625-02, R01 NS050625-04] Funding Source: Medline
Understanding the mechanisms that govern neuronal responses to oxidative and metabolic stress is essential for therapeutic intervention. In vitro modeling is an important approach for these studies, as the metabolic environment influences neuronal responses. Surprisingly, most neuronal culture methods employ conditions that are non-physiological, especially with regards to glucose concentrations, which often exceed 20 mM. This concentration is a significant departure from physiological glucose levels, and even several-fold greater than that seen during severe hyperglycemia. The goal of this study was to establish a physiological neuronal culture system that will facilitate the study of neuronal energy metabolism and responses to metabolic stress. We demonstrate that the metabolic environment during preparation, plating, and maintenance of cultures affects neuronal viability and the response of neuronal pathways to changes in energy balance. (c) 2007 Elsevier B.V. All rights reserved.
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