期刊
JOURNAL OF NEUROSCIENCE
卷 34, 期 35, 页码 11781-11791出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1444-14.2014
关键词
glutamate; hippocampus; release probability; synaptic transmission; vesicular transporter
资金
- Alexander von Humboldt Foundation [1141215]
- European Research Council (Advanced Grant SYNVGLUT)
- German Excellence Initiative [Exc 257]
The vesicular glutamate transporter (VGLUT) plays an essential role in synaptic transmission by filling vesicles with glutamate. At mammalian synapses, VGLUT expression level determines the amount of glutamate packaged into vesicles, and the specific paralog of VGLUT expressed affects the release probability. In this study, we investigate whether there is a link between the number of VGLUTs on vesicles and release probability. We used a combination of electrophysiology and imaging techniques in cultured mouse hippocampal neurons where the VGLUT expression level has been severely altered. We found that vesicles with drastically reduced VGLUT expression were released with a lower probability. This deficit in release could only be rescued by a functional transporter, suggesting that the transport function, and not the molecular interactions, of the protein affects vesicle release. Based on these data, we propose a novel means of presynaptic vesicle release regulation-the intravesicular glutamate fill state of the vesicle.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据