期刊
JOURNAL OF NEUROSCIENCE
卷 34, 期 50, 页码 16594-16604出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2585-14.2014
关键词
adenosine; EEG; GFAP; sleep; SNARE
资金
- National Institutes of Health [R01DE022743, R01NS075177, R01AT007945]
- Novo Nordisk Fonden [NNF13OC0004258] Funding Source: researchfish
- Direct For Social, Behav & Economic Scie
- SBE Off Of Multidisciplinary Activities [1344466] Funding Source: National Science Foundation
Experimental advances in the study of neuroglia signaling have been greatly accelerated by the generation of transgenic mouse models. In particular, an elegant manipulation that interferes with astrocyte vesicular release of gliotransmitters via overexpression of a dominant-negative domain of vesicular SNARE (dnSNARE) has led to documented astrocytic involvement in processes that were traditionally considered strictly neuronal, including the sleep-wake cycle, LTP, cognition, cortical slow waves, depression, and pain. A key premise leading to these conclusions was that expression of the dnSNARE was specific to astrocytes. Inconsistent with this premise, we report here widespread expression of the dnSNARE transgene in cortical neurons. We further demonstrate that the activity of cortical neurons is reversibly suppressed in dnSNARE mice. These findings highlight the need for independent validation of astrocytic functions identified in dnSNARE mice and thus question critical evidence that astrocytes contribute to neurotransmission through SNARE-dependent vesicular release of gliotransmitters.
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