期刊
JOURNAL OF NEUROSCIENCE
卷 34, 期 43, 页码 14375-14387出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1222-14.2014
关键词
Barnes maze; cortical development; Huntington disease; ppargc1a; schizophrenia; strontium
资金
- National Institutes of Health (NIH) [K01MH077955-05, R01NS070009-05, P30NS047466, P30HD038985]
- Civitan McNulty Investigator awards
- NIH-NCRR [RR12546]
Accumulating evidence strongly implicates the transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1 alpha) in the pathophysiology of multiple neurological disorders, but the downstream gene targets of PGC-1 alpha in the brain have remained enigmatic. Previous data demonstrate that PGC-1 alpha is primarily concentrated in inhibitory neurons and that PGC-1 alpha is required for the expression of the interneuron-specific Ca2+-binding protein parvalbumin (PV) throughout the cortex. To identify other possible transcriptional targets of PGC-1 alpha in neural tissue, we conducted a microarray on neuroblastoma cells overexpressing PGC-1 alpha, mined results for genes with physiological relevance to interneurons, and measured cortical gene and protein expression of these genes in mice with underexpression and overexpression of PGC-1 alpha. We observed bidirectional regulation of novel PGC-1 alpha-dependent transcripts spanning synaptic [synaptotagmin 2 (Syt2) and complexin 1 (Cplx1)], structural [neurofilament heavy chain (Nefh)], and metabolic [neutral cholesterol ester hydrolase 1 (Nceh1), adenylate kinase 1 (Ak1), inositol polyphosphate 5-phosphatase J (Inpp5j), ATP synthase mitochondrial F1 complex 0 subunit (Atp5o), phytanol-CoA-2hydroxylase (Phyh), and ATP synthase mitrochondrial F1 complex alpha subunit 1 (Atp5a1)] functions. The neuron-specific genes Syt2, Cplx1, and Nefh were developmentally upregulated in an expression pattern consistent with that of PGC-1 alpha and were expressed in cortical interneurons. Conditional deletion of PGC-1 alpha in PV-positive neurons significantly decreased cortical transcript expression of these genes, promoted asynchronous GABA release, and impaired long-term memory. Collectively, these data demonstrate that PGC-1 alpha is required for normal PV-positive interneuron function and that loss of PGC-1 alpha in this interneuron subpopulation could contribute to cortical dysfunction in disease states.
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