期刊
JOURNAL OF NEUROSCIENCE
卷 34, 期 9, 页码 3268-3278出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4903-13.2014
关键词
olfactory; pheromone; transduction; TRPM5
资金
- MIDEPLAN [ICM-P05-001]
- Fondo Nacional de Desarrollo Cient fico y Tecnologico (FONDECYT) [1100682, DC04657, DC006070, TW007920]
Growing evidence suggests that the main olfactory epithelium contains a subset of olfactory sensory neurons (OSNs) responding to pheromones. One candidate subpopulation expresses the calcium activated cation channel TRPM5 (transient receptor potential channel M5). Using GFP driven by the TRPM5 promoter in mice, we show that this subpopulation responds to putative pheromones, urine, and major histocompatibility complex peptides, but not to regular odors or a pheromone detected by other species. In addition, this subpopulation of TRPM5-GFP(+) OSNs uses novel transduction. In regular OSNs, odorants elicit activation of the cyclic nucleotide-gated (CNG) channel, leading to Ca2+ gating of Cl- channels; in TRPM5-GFP(+) OSNs, the Ca2+-activated Cl- ANO2 (anoctamin 2) channel is not expressed, and pheromones elicit activation of the CNG channel leading to Ca2+ gating of TRPM5. In conclusion, we show that OSNs expressing TRPM5 respond to pheromones, but not to regular odors through the opening of CNG channels leading to Ca2+ gating of TRPM5.
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