期刊
JOURNAL OF NEUROSCIENCE
卷 34, 期 16, 页码 5416-5430出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3183-13.2014
关键词
Drosophila; electrophysiology; Frazzled; gap junctions; giant fiber system; Netrin
资金
- Florida Atlantic University Research Priority Grant from the Office of the Vice President for Research
Netrin and its receptor, Frazzled, dictate the strength of synaptic connections in the giant fiber system (GFS) of Drosophila melanogaster by regulating gap junction localization in the presynaptic terminal. In Netrin mutant animals, the synaptic coupling between a giant interneuron and the jump motor neuron was weakened and dye coupling between these two neurons was severely compromised or absent. In cases in which Netrin mutants displayed apparently normal synaptic anatomy, half of the specimens exhibited physiologically defective synapses and dye coupling between the giant fiber (GF) and the motor neuron was reduced or eliminated, suggesting that gap junctions were disrupted in the Netrin mutants. Whenwe examined the gap junctions with antibodies to Shaking-B (ShakB) Innexin, they were significantly decreased or absent in the presynaptic terminal of the mutant GF. Frazzled loss of function mutants exhibited similar defects in synaptic transmission, dye coupling, and gap junction localization. These data are the first to show that Netrin and Frazzled regulate the placement of gap junctions presynaptically at a synapse.
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