期刊
JOURNAL OF NEUROSCIENCE
卷 34, 期 18, 页码 6128-6139出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4941-13.2014
关键词
glutamate sensor; kainate receptor; mouse; OFF bipolar cell; retinal circuitry; two-photon imaging
资金
- NIH/NEI [R01 EY014454, R21 EY023038]
- NIH/NIMH [R01 MH085080]
- Howard Hughes Medical Institute
- Research to Prevent Blindness to Yale University
A fundamental question in sensory neuroscience is how parallel processing is implemented at the level of molecular and circuit mechanisms. In the retina, it has been proposed that distinct OFF cone bipolar cell types generate fast/transient and slow/sustained pathways by the differential expression of AMPA-and kainate-type glutamate receptors, respectively. However, the functional significance of these receptors in the intact circuit during light stimulation remains unclear. Here, we measured glutamate release from mouse bipolar cells by two-photon imaging of a glutamate sensor (iGluSnFR) expressed on postsynaptic amacrine and ganglion cell dendrites. In both transient and sustained OFF layers, cone-driven glutamate release from bipolar cells was blocked by antagonists to kainate receptors but not AMPA receptors. Electrophysiological recordings from bipolar and ganglion cells confirmed the essential role of kainate receptors for signaling in both transient and sustained OFF pathways. Kainate receptors mediated responses to contrast modulation up to 20 Hz. Light-evoked responses in all mouse OFF bipolar pathways depend on kainate, not AMPA, receptors.
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