期刊
JOURNAL OF NEUROSCIENCE
卷 33, 期 17, 页码 7206-7219出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.5721-12.2013
关键词
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资金
- German Israeli Foundation [917/2006]
- Hezneq faculty grant
- NeuroCure Cluster of Excellence (Charite University Hospital, Berlin)
- National Science Foundation [CBET 0943343]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [943343] Funding Source: National Science Foundation
Mitochondria not only provide cells with energy, but are central to Ca2+ signaling. Powered by the mitochondrial membrane potential, Ca2+ enters the mitochondria and is released into the cytosol through a mitochondrial Na+/Ca2+ exchanger. We established that NCLX, a newly discovered mitochondrial Na+/Ca2+ exchanger, is expressed in astrocytes isolated from mice of either sex. Immunoblot analysis of organellar fractions showed that the location of NCLX is confined to mitochondria. Using pericam-based mitochondrial Ca2+ imaging and NCLX inhibition either by siRNA or by the pharmacological blocker CGP37157, we demonstrated that NCLX is responsible for mitochondrial Ca2+ extrusion. Suppression of NCLX function altered cytosolic Ca2+ dynamics in astrocytes and this was mediated by a strong effect of NCLX activity on Ca2+ influx via store-operated entry. Furthermore, Ca2+ influx through the store-operated Ca2+ entry triggered strong, whereas ER Ca2+ release triggered only modest mitochondrial Ca2+ transients, indicating that the functional cross talk between the plasma membrane and mitochondrial domains is particularly strong in astrocytes. Finally, silencing of NCLX expression significantly reduced Ca2+-dependent processes in astrocytes (i.e., exocytotic glutamate release, in vitro wound closure, and proliferation), whereas Ca2+ wave propagation was not affected. Therefore, NCLX, by meditating astrocytic mitochondrial Na+/Ca2+ exchange, links between mitochondria and plasma membrane Ca2+ signaling, thereby modulating cytoplasmic Ca2+ transients required to control a diverse array of astrocyte functions.
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