期刊
JOURNAL OF NEUROSCIENCE
卷 33, 期 8, 页码 3402-3412出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3192-12.2013
关键词
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资金
- National Institutes of Health [DA10355, MH61469]
- Saint Luke's Hospital Foundation
The metabotropic glutamate receptor 1 (mGluR1) is a G alpha(q)-protein-coupled receptor and is distributed in broad regions of the mammalian brain. As a key element in excitatory synaptic transmission, the receptor regulates a wide range of cellular and synaptic activities. In addition to regulating its targets, the receptor itself is believed to be actively regulated by intracellular signals, although underlying mechanisms are essentially unknown. Here we found that a synapse-enriched protein kinase, Ca2+/calmodulin-dependent protein kinase II alpha (CaMKII alpha), directly binds to the intracellular C terminus (CT) of mGluR1a. This binding is augmented by Ca2+ in vitro. The direct interaction promotes CaMKII alpha to phosphorylate mGluR1a at a specific threonine site (T871). In rat striatal neurons, the mGluR1 agonist triggers the receptor-associated phosphoinositide signaling pathway to induce Caa(2+)-dependent recruitment of CaMKII alpha to mGluR1a-CT. This enables the kinase to inhibit the response of the receptor to subsequent agonist exposure. Our data identify an agonist-induced and Ca2+-dependent protein-protein interaction between a synaptic kinase and mGluR1, which constitutes a feedback loop facilitating desensitization of mGluR1a.
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