期刊
JOURNAL OF NEUROSCIENCE
卷 33, 期 1, 页码 35-51出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.0245-12.2013
关键词
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资金
- Howard Hughes Medical Institute
- National Institutes of Health (NIH) [NS068012-01]
- NIH Grant [R01 EY014454, R01 EY022157-01]
- Early Career Award from The Ziegler Foundation for the Blind
- Glaucoma Research Foundation Catalyst for a Cure Initiative
The use of neurotropic viruses as transsynaptic tracers was first described in the 1960s, but only recently have such viruses gained popularity as a method for labeling neural circuits. The development of retrograde monosynaptic tracing vectors has enabled visualization of the presynaptic sources onto defined sets of postsynaptic neurons. Here, we describe the first application of a novel viral tracer, based on vesicular stomatitis virus (VSV), which directs retrograde transsynaptic viral spread between defined cell types. We use this virus in the mouse retina to show connectivity between starburst amacrine cells (SACs) and their known synaptic partners, direction-selective retinal ganglion cells, as well as to discover previously unknown connectivity between SACs and other retinal ganglion cell types. These novel connections were confirmed using physiological recordings. VSV transsynaptic tracing enables cell type-specific dissection of neural circuitry and can reveal synaptic relationships among neurons that are otherwise obscured due to the complexity and density of neuropil.
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