期刊
JOURNAL OF NEUROSCIENCE
卷 32, 期 10, 页码 3321-3332出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.5341-11.2012
关键词
-
资金
- NIH [EY140701]
In the retina, the receptive fields (RFs) of almost all ganglion cells (GCs) are comprised of an excitatory center and a suppressive surround. The RF center arises from local excitatory bipolar cell ( BC) inputs and the surround from lateral inhibitory inputs. Selective antagonists have been used to define the roles of GABA(A) and GABA(C) receptor-mediated input in RF organization. In contrast, the role of glycine receptor ( GlyR) subunit-specific inhibition is less clear because the only antagonist, strychnine, blocks all GlyR subunit combinations. We used mice lacking the GlyR alpha 2 (Glra2(-/-)) and GlyR alpha 3 (Glra3(-/-)) subunits, or both (Glra2/3(-/-)), to explore their roles in GC RF organization. By comparing spontaneous and visually evoked responses of WT with Glra2(-/-), Glra3(-/-) and Glra2/3(-/-) ON- and OFF-center GCs, we found that both GlyR alpha 2 and GlyR alpha 3 modulate local RF interactions. In the On pathway, both receptors enhance the excitatory center response; however, the underlying inhibitory mechanisms differ. GlyR alpha 2 participates in crossover inhibition, whereas GlyR alpha 3 mediates serial inhibition. In the Off pathway, GlyR alpha 2 plays a similar role, again using crossover inhibition and enhancing excitatory responses within the RF center. Comparisons of single and double KOs indicate that GlyR alpha 2 and GlyR alpha 3 inhibition are independent and additive, consistent with the finding that they use different inhibitory circuitry. These findings are the first to define GlyR subunit-specific control of visual function and GlyR alpha 2 subunit-specific control of crossover inhibition in the retina.
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