期刊
JOURNAL OF NEUROSCIENCE
卷 31, 期 6, 页码 2216-2224出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2653-10.2011
关键词
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资金
- Ministry of Education, Culture and Science of Japan
- The Sumitomo Foundation
- The Takeda Foundation
- Council of Scientific and Industrial Research
- Department of Biotechnology, Ministry of Science and Technology
- Grants-in-Aid for Scientific Research [21247031, 23590331] Funding Source: KAKEN
Kinesin-1 is a microtubule plus-end-directed motor that transports various cargos along the axon. Previous studies have elucidated the physical and genetic interactions between kinesin-1 and cytoplasmic dynein, a microtubule minus-end-directed motor, in neuronal cells. However, the physiological importance of kinesin-1 in the dynein-dependent retrograde transport of cargo molecules remains obscure. Here, we show that Caenorhabditis elegans kinesin-1 forms a complex with dynein via its interaction with UNC-16, which binds to the dynein light intermediate (DLI) chain. Both lcinesin-1 and UNC-16 are required for localization of DLI-1 at the plus ends of nerve process microtubules. In addition, retrograde transport of APL-1 depends on kinesin-1, UNC-16, and dynein. These results suggest that kinesin-1 mediates the anterograde transport of dynein using UNC-16 as a scaffold and that dynein in turn mediates the retrograde transport of cargo molecules in vivo. Thus, UNC-16 functions as an adaptor for kinesin-1-mediated transport of dynein.
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