期刊
JOURNAL OF NEUROSCIENCE
卷 29, 期 46, 页码 14553-14570出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3287-09.2009
关键词
-
资金
- National Institutes of Health [EB003832, MH72570, MH085499, NS059832, NS43300, NS052565, DC006849]
- Israeli Science Foundation
It is well known that the density of neurons varies within the adult brain. In neocortex, this includes variations in neuronal density between different lamina as well as between different regions. Yet the concomitant variation of the microvessels is largely uncharted. Here, we present automated histological, imaging, and analysis tools to simultaneously map the locations of all neuronal and non-neuronal nuclei and the centerlines and diameters of all blood vessels within thick slabs of neocortex from mice. Based on total inventory measurements of different cortical regions (similar to 10(7) cells vectorized across brains), these methods revealed: ( 1) In three dimensions, the mean distance of the center of neuronal somata to the closest microvessel was 15 mu m. ( 2) Volume samples within lamina of a given region show that the density of microvessels does not match the strong laminar variation in neuronal density. This holds for both agranular and granular cortex. ( 3) Volume samples in successive radii from the midline to the ventral-lateral edge, where each volume summed the number of cells and microvessels from the pia to the white matter, show a significant correlation between neuronal and microvessel densities. These data show that while neuronal and vascular densities do not track each other on the 100 mu m scale of cortical lamina, they do track each other on the 1-10 mm scale of the cortical mantle. The absence of a disproportionate density of blood vessels in granular lamina is argued to be consistent with the initial locus of functional brain imaging signals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据