期刊
JOURNAL OF NEUROSCIENCE
卷 29, 期 4, 页码 918-929出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3952-08.2009
关键词
nicotinic receptor; basal forebrain; cholinergic neurons; patch clamp; amyloid beta; Alzheimer's disease
资金
- Arizona Alzheimer's Consortium Pilot
- Barrow Neurological Foundation
- National Institutes of Health [R01 DA015389]
Nicotinic acetylcholine receptors (nAChRs) containing alpha 7 subunits are thought to assemble as homomers. alpha 7-nAChR function has been implicated in learning and memory, and alterations of alpha 7-nAChR have been found in patients with Alzheimer's disease (AD). Here we report findings consistent with a novel, naturally occurring nAChR subtype in rodent, basal forebrain cholinergic neurons. In these cells, alpha 7 subunits are coexpressed, colocalize, and coassemble with beta 2 subunit(s). Compared with homomeric alpha 7-nAChRs from ventral tegmental area neurons, functional, presumably heteromeric alpha 7 beta 2-nAChRs on cholinergic neurons freshly dissociated from medial septum/diagonal band (MS/DB) exhibit relatively slow kinetics of whole-cell current responses to nicotinic agonists and are more sensitive to the beta 2 subunit-containing nAChR-selective antagonist, dihydro-beta-erythroidine (DH beta E). Interestingly, presumed, heteromeric alpha 7 beta 2-nAChRs are highly sensitive to functional inhibition by pathologically relevant concentrations of oligomeric, but not monomeric or fibrillar, forms of amyloid beta(1-42) (A beta(1-42)). Slow whole-cell current kinetics, sensitivity to DH beta E, and specific antagonism by oligomericA beta(1-42) also are characteristics of heteromeric alpha 7 beta 2-nAChRs, but not of homomeric alpha 7-nAChRs, heterologously expressed in Xenopus oocytes. Moreover, choline-induced currents have faster kinetics and less sensitivity to A beta when elicited from MS/DB neurons derived from nAChR beta 2 subunit knock-out mice rather than from wild-type mice. The presence of novel, functional, heteromeric alpha 7 beta 2-nAChRs on basal forebrain cholinergic neurons and their high sensitivity to blockade by low concentrations of oligomeric A beta(1-42) suggests possible mechanisms for deficits in cholinergic signaling that could occur early in the etiopathogenesis of AD and might be targeted by disease therapies.
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