期刊
JOURNAL OF NEUROSCIENCE
卷 28, 期 10, 页码 2301-2312出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.5157-07.2008
关键词
cerebellum; cell lineage; granule cells; clonal analysis; neurogenesis; MADM
资金
- Howard Hughes Medical Institute Funding Source: Medline
- NINDS NIH HHS [F31 NS053270-03, R01 NS050835, F31 NS053270-01, R01-NS050835, F31 NS053270-02, F31 NS053270] Funding Source: Medline
The cerebellum is an excellent model system to study how developmental programs give rise to exquisite neuronal circuits in the adult brain. Here, we describe our findings regarding granule cell neurogenesis and differentiation using the MADM method (mosaic analysis with double markers) in mice. By following the development of individual granule cell clones, we show that (1) granule cell precursors (GCPs) undergo predominantly symmetric division during postnatal development; (2) clonally related granule cells (GCs) exit the cell cycle within a narrow time window and stack their axons in the molecular layer in chronological order from deep to superficial sublayers; and (3) whereas the average GCP proliferation in the external granular layer is progressively slower as development proceeds, there is a rapid expansion of GCPs shortly before clonally related GCs exit the cell cycle. These properties produce GC clones that are distinct, each having a restricted axonal projection, but that are on average similar in cell number. We discuss possible developmental mechanisms and functional implications of these findings.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据