期刊
JOURNAL OF NEUROSCIENCE
卷 28, 期 42, 页码 10472-10481出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2537-08.2008
关键词
neurofibromatosis type 2; nodes of Ranvier; paranodes; juxtaparanodes; Schmidt-Lanterman incisures; internodes
资金
- National Multiple Sclerosis Society
- Association pour la Recherche sur la Sclerose en Plaque
- Association Francaise contre les Myopathies
- Action Concertee Incitative Developpement et Physiologie
- Association pour la Recherche sur le Cancer
- Foundation Schlumberger pour l'enseignement et la Recherche
- Agence Nationale de la Recherche [Neuro-05-NEUR-A05158DS]
Schwannomin/merlin is the product of a tumor suppressor gene mutated in neurofibromatosis type 2 (NF2). Although the consequences of NF2 mutations on Schwann cell proliferation are well established, the physiological role of schwannomin in differentiated cells is not known. To unravel this role, we studied peripheral nerves in mice overexpressing in Schwann cells schwannomin with a deletion occurring in NF2 patients (P0-SCH-Delta 39-121) or a C-terminal deletion. The myelin sheath and nodes of Ranvier were essentially preserved in both lines. In contrast, the ultrastructural and molecular organization of contacts between Schwann cells and axons in paranodal and juxtaparanodal regions were altered, with irregular juxtaposition of normal and abnormal areas of contact. Similar but more severe alterations were observed in mice with conditional deletion of the Nf2 gene in Schwann cells. The number of Schmidt Lanterman incisures, which are cytoplasmic channels interrupting the compact myelin and characterized by distinct autotypic contacts, was increased in the three mutant lines. P0-SCH-Delta 39-121 and conditionally deleted mice displayed exuberant wrapping of nonmyelinated fibers and short internodes, an abnormality possibly related to altered control of Schwann cell proliferation. In support of this hypothesis, Schwann cell number was increased along fibers before myelination in P0-SCH-Delta 39-121 mice but not in those with C-terminal deletion. Schwann cell numbers were also more numerous in mice with conditional deletion. Thus, schwannomin plays an important role in the control of Schwann cell number and is necessary for the correct organization and regulation of axoglial heterotypic and glio-glial autotypic contacts.
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