期刊
JOURNAL OF NEUROSCIENCE
卷 28, 期 1, 页码 106-115出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.3996-07.2008
关键词
alpha(4) subunit; tonic inhibition; synaptic transmission; VB; ion channel; glycine receptor
资金
- NIAAA NIH HHS [R01 AA013004, AA 16393, R01 AA016393, AA 13004] Funding Source: Medline
- NIGMS NIH HHS [P01 GM047818, R01 GM066840, GM 066840, GM 47818] Funding Source: Medline
Taurine is one of the most abundant free amino acids in the brain. In a number of studies, taurine has been reported to activate glycine receptors (Gly-Rs) at moderate concentrations (>= 100 mu M), and to be a weak agonist at GABA(A) receptors (GABA(A)-Rs), which are usually activated at high concentrations (>= 1 mM). In this study, we show that taurine reduced the excitability of thalamocortical relay neurons and activated both extrasynaptic GABAA-Rs and Gly-Rs in neurons in the mouse ventrobasal (VB) thalamus. Low concentrations of taurine (10 - 100 mu M) decreased neuronal input resistance and firing frequency, and elicited a steady outward current under voltage clamp, but had no effects on fast inhibitory synaptic currents. Currents elicited by 50 mu M taurine were abolished by gabazine, insensitive to midazolam, and partially blocked by 20 mu M Zn2+, consistent with the pharmacological properties of extrasynaptic GABA(A)-Rs (alpha 4 beta 2 delta subtype) involved in tonic inhibition in the thalamus. Tonic inhibition was enhanced by an inhibitor of taurine transport, suggesting that taurine can act as an endogenous activator of these receptors. Taurine-evoked currents were absent in relay neurons from GABA(A)-R alpha 4 subunit knock-out mice. The amplitude of the taurine current was larger in neurons from adult mice than juvenile mice. Taurine was a more potent agonist at recombinant alpha(4)beta 2 delta GABA(A)-Rs than at alpha 1 beta 2 gamma 2 GABA(A)-Rs. We conclude that physiological concentrations of taurine can inhibit VB neurons via activation of extrasynaptic GABA(A)-Rs and that taurine may function as an endogenous regulator of excitability and network activity in the thalamus.
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