期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 99, 期 2, 页码 976-988出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00930.2007
关键词
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资金
- NIDA NIH HHS [R21 DA016352, R21 DA016352-02, DA-016352] Funding Source: Medline
- NINDS NIH HHS [R01 NS027501, R56 NS027501, R01 NS046750-04, R01 NS046750, NS-046750] Funding Source: Medline
- Direct For Biological Sciences [0757242] Funding Source: National Science Foundation
- Division Of Integrative Organismal Systems [0757242] Funding Source: National Science Foundation
The ventral lateral neurons ( LNvs) of adult Drosophila brain express oscillating clock proteins and regulate circadian behavior. Whole cell current-clamp recordings of large LNvs in freshly dissected Drosophila whole brain preparations reveal two spontaneous activity patterns that correlate with two underlying patterns of oscillating membrane potential: tonic and burst firing of sodium-dependent action potentials. Resting membrane potential and spontaneous action potential firing are rapidly and reversibly regulated by acute changes in light intensity. The LNv electrophysiological light response is attenuated, but not abolished, in cry(b) mutant flies hypomorphic for the cell-autonomous light-sensing protein CRYPTOCHROME. The electrical activity of the large LNv is circadian regulated, as shown by significantly higher resting membrane potential and frequency of spontaneous action potential firing rate and burst firing pattern during circadian subjective day relative to subjective night. The circadian regulation of membrane potential, spontaneous action potential firing frequency, and pattern of Drosophila large LNvs closely resemble mammalian circadian neuron electrical characteristics, suggesting a general evolutionary conservation of both physiological and molecular oscillator mechanisms in pacemaker neurons.
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