期刊
JOURNAL OF NEUROPHYSIOLOGY
卷 100, 期 4, 页码 2361-2380出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.90332.2008
关键词
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资金
- SFB-TR3 funding [C2, C7]
- Deutsche Forschungsgemeinschaft [LE660/4-1]
- Ministry of Science, Israel
- Humboldt Foundation
- BONFOR
In many neuron types, the axon initial segment (AIS) has the lowest threshold for action potential generation. Its active properties are determined by the targeted expression of specific voltage-gated channel subunits. We show that the Na+ channel Na(V)1.6 displays a striking aggregation at the AIS of cortical neurons. To assess the functional role of this subunit, we used Scn8a(med) mice that are deficient for Na(V)1.6 subunits but still display prominent Na+ channel aggregation at the AIS. In CA1 pyramidal cells from Scn8a(med) mice, we found a depolarizing shift in the voltage dependence of activation of the transient Na+ current (I-NaT), indicating that Na(V)1.6 subunits activate at more negative voltages than other Na-V subunits. Additionally, persistent and resurgent Na+ currents were significantly reduced. Current-clamp recordings revealed a significant elevation of spike threshold in Scn8a(med) mice as well as a shortening of the estimated delay between spike initiation at the AIS and its arrival at the soma. In combination with simulations using a realistic computer model of a CA1 pyramidal cell, our results imply that a hyperpolarized voltage dependence of activation of AIS Na(V)1.6 channels is important both in determining spike threshold and localizing spike initiation to the AIS. In addition to altered spike initiation, Scn8a(med) mice also showed a strongly reduced spike gain as expected with combined changes in persistent and resurgent currents and spike threshold. These results suggest that Na(V)1.6 subunits at the AIS contribute significantly to its role as spike trigger zone and shape repetitive discharge properties of CA1 neurons.
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