期刊
JOURNAL OF NEUROIMMUNOLOGY
卷 265, 期 1-2, 页码 96-105出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneuroim.2013.09.016
关键词
HIV-associated neurocognitive disorder transcriptome; Monocyte; IL6R; gene expression; WGCNA
资金
- National Institute for Drug Abuse [R01DA030913]
- National Institute of Allergy and Infectious Disease [U01-AI-35040]
- NIH/National Center for Advancing Translational Science (NCATS) UCLA CTSI [UL1TR000124]
- NIH [AI-28697]
Immunologic dysfunction, mediated via monocyte activity, has been implicated in the development of HIV-associated neurocognitive disorder (HAND). We hypothesized that transcriptome changes in peripheral blood monocytes relate to neurocognitive functioning in HIV+ individuals, and that such alterations could be useful as biomarkers of worsening HAND. Methods: mRNA was isolated from the monocytes of 86 HIV+ adults and analyzed with the Illumina HT-12 v4 Expression BeadChip. Neurocognitive functioning, HAND diagnosis, and other clinical and virologic variables were determined. Data were analyzed using standard expression analysis and weighted gene co-expression network analysis (WGCNA). Results: Neurocognitive functioning was correlated with multiple gene transcripts in the standard expression analysis. WGCNA identified two nominally significant co-expression modules associated with neurocognitive functioning, which were enriched with genes involved in mitotic processes and translational elongation. Conclusions: Multiple modified gene transcripts involved in inflammation, cytoprotection, and neurodegeneration were correlated with neurocognitive functioning. The associations were not strong enough to justify their use as biomarkers of HAND; however, the associations of two co-expression modules with neurocognitive functioning warrant further exploration. (C) 2013 Elsevier B.V. All rights reserved.
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