期刊
JOURNAL OF NEUROIMMUNOLOGY
卷 208, 期 1-2, 页码 80-86出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneuroim.2009.01.008
关键词
DRG neurons; Immunoglobulin-free light chain; Intracellular calcium
Immunoglobulin-free light chains (IgLC) secreted by B lymphocytes, have been shown to mediate hypersensitivity by inducing antigen-specific mast cell activation. Although both mast cells and sensory neurons contribute to the hypersensitivity response. the role of IgLC in relation to sensory neurons is unknown. We therefore aimed to investigate the effects of IgLC on cultures of murine dorsal root ganglion (DRG) neurons. Immunohistochemistry demonstrated that lgLC and IgE could specifically bind to DRG neurons, on which the presence of Fc epsilon RI, the specific receptor for IgE, was demonstrated by western blotting. Further, optical recordings with Fluo-4 showed that application of the corresponding antigen to IgLC- or IgE-sensitized DRG neurons induces a sustained increase in intracellular Ca2+ in about half of these neurons. These results show that IgLC and IgE can mediate antigen-specific responses in murine neurons. Our findings present a novel way of antigen-specific neuronal activation. (C) 2009 Elsevier B.V. All rights reserved.
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