期刊
PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY
卷 12, 期 1, 页码 131-135出版社
ELSEVIER
DOI: 10.1016/j.pdpdt.2014.10.002
关键词
Antimicrobial photodynamic inactivation; Antimicrobial photodynamic chemotherapy; Periodontitis; Periodontal disease; Photosensitizer; Red laser
类别
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [05/01756-5, 10/13313-9]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [10/13313-9, 05/01756-5] Funding Source: FAPESP
Background: The purpose of this study was to evaluate the antibacterial effects of photodynamic action of methylene blue (MB) against Aggregatibacter actinomycetemcomitans organized on biofilm. Methods: After the biofilm growth in 96 flat-bottom well plate, the following groups were used: control group, untreated by either laser or photosensitizer (PS); MB group or dark toxicity group, which was exposed to MB atone (100 mu M) for 1 min (pre-irradiation time); laser group, irradiated with laser for 5 min in the absence of PS and three antimicrobial photodynamic inactivation (APDI) groups, with three exposure times of 1, 3 and 5 min of irradiation, corresponding to fluences of 15, 45, and 75 J/cm(2) respectively. The results were compared to the control group for statistical proposes. Scanning electronic microscope analysis was used to access structural changes in biofilm. Results: Red laser alone and MB alone were not able to inactivate bacterial biofilm. APDI groups showed differences when compared to the control group and they were dependent on the exposure time. No statistically significant differences were observed among the APDI groups at 1 and 3 min of irradiation. On the other hand, 5 min of APDI showed 99.85% of bacterial reduction (p=0.0004). In addition, the biofilm loose its structure following 5 min APDI. Conclusions: The results of this study suggest that A. actinomycetemcomitans biofilm can be inactivated by MB mediated APDI. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据