期刊
JOURNAL OF NEUROCHEMISTRY
卷 124, 期 2, 页码 168-174出版社
WILEY
DOI: 10.1111/jnc.12053
关键词
activation; microglia; prion protein; siRNA
资金
- Natural Science Foundation of China [31001048, 31172293]
- Specialized Research Fund for the Doctoral Program of Higher Education (SRFDP) [20100008120002]
- Foundation of Chinese Ministry of Science and Technology [2011BAI15B01]
- Program for Cheung Kong Scholars and Innovative Research Team in University of China [IRT0866]
The cellular prion protein (PrPC) is a glycoprotein anchored by glycosylphosphatidylinositol (GPI) to the cell surface and is abundantly expressed in the central nervous system. Numerous studies have suggested a protective function for PrPC, including protection from ischemic and excitotoxic lesions and several apoptotic insults, and recent reports have shown that PrPC has a context-dependent neuroprotective function. In this study, we investigated the effect of PPNP down-regulation on various forms of microglial activation. We first examined the mRNA expression of PRNP upon exposure to IFN-gamma, IL-4, or IL-10 in BV2 microglia. We then analyzed the effect of si-RNA-mediated disruption of PRNP on different parameters of microglial activation in IFN-gamma-, IL-4-, or IL-10-stimulated microglia. The results showed that PRNP mRNA expression was invariably down-regulated in microglia upon exposure to IFN-gamma, IL-4, or IL-10. PRNP silencing prior to cytokines treatment reduced the responsiveness of microglia to INF-gamma treatment, significantly altered IL-4-induced microglial activation phenotype, and had no effect on IL-10-induced microglial activation. Together, these results support a role of PrPC in the modulation of the shift of microglia from a quiescent state to an activated phenotype and in the regulation of the microglial response during classical and alternative activation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据