期刊
JOURNAL OF NEUROCHEMISTRY
卷 114, 期 6, 页码 1630-1638出版社
WILEY
DOI: 10.1111/j.1471-4159.2010.06888.x
关键词
Alzheimer's disease; ATP7A; copper homeostasis; inflammation; microglia
资金
- NIH [DK59893, AG018357]
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK059893, R01DK079209] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [P01AG018357] Funding Source: NIH RePORTER
P>Alzheimer's disease (AD) is characterized by progressive neurodegeneration associated with the aggregation and deposition of beta-amyloid (A beta(40) and A beta(42)) peptide in senile plaques. Recent studies suggest that copper may play an important role in AD pathology. Copper concentrations are elevated in amyloid plaques and copper binds with high affinity to the A beta peptide and promotes A beta oligomerization and neurotoxicity. Despite this connection between copper and AD, it is unknown whether the expression of proteins involved in regulating copper homeostasis is altered in this disorder. In this study, we demonstrate that the copper transporting P-type ATPase, ATP7A, is highly expressed in activated microglial cells that are specifically clustered around amyloid plaques in the TgCRND8 mouse model of AD. Using a cultured microglial cell line, ATP7A expression was found to be increased by the pro-inflammatory cytokine interferon-gamma, but not by TNF-alpha or IL-1 beta. Interferon-gamma also elicited marked changes in copper homeostasis, including copper-dependent trafficking of ATP7A from the Golgi to cytoplasmic vesicles, increased copper uptake and elevated expression of the CTR1 copper importer. These findings suggest that pro-inflammatory conditions associated with AD cause marked changes in microglial copper trafficking, which may underlie the changes in copper homeostasis in AD. It is concluded that copper sequestration by microglia may provide a neuroprotective mechanism in AD.
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