4.5 Article

mGluR1 antagonist decreased NADPH oxidase activity and superoxide production after transient focal cerebral ischemia

期刊

JOURNAL OF NEUROCHEMISTRY
卷 114, 期 6, 页码 1711-1719

出版社

WILEY-BLACKWELL
DOI: 10.1111/j.1471-4159.2010.06882.x

关键词

cerebral ischemia; metabotropic glutamate receptor; NADPH oxidase; p67phox

资金

  1. Japan Society for the Promotion of Science

向作者/读者索取更多资源

NADPH oxidase, which is activated by PKC and signaling via the NMDA receptor, is one of the crucial enzymes for superoxide production in the CNS. We showed earlier that the metabotropic glutamate receptor 1 (mGluR1) plays an important role in the activation of PKC and tyrosine phosphorylation of the NMDA receptor, which has been implicated in enhancement of the channel activity, after cerebral ischemia. In this study, we sought to determine the role of mGluR1 in the activation of NADPH oxidase and subsequent superoxide production after transient focal cerebral ischemia. The amounts of NADPH oxidase subunits in the membrane fraction were increased after the start of reperfusion. These changes were accompanied by increased NADPH oxidase activity followed by superoxide production. The administration of an mGluR1 antagonist attenuated NADPH oxidase activity, which was coincident with inhibition of superoxide production. We further showed that the increase in the amount of PKC delta, but not of PKC zeta, as well as the increase in those of NADPH oxidase subunits, was attenuated by the mGluR1 antagonist. These results suggest that mGluR1 may be linked to the increase in NADPH oxidase activity that is mediated by PKC delta and subsequent superoxide production after cerebral ischemia.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据