期刊
JOURNAL OF NEUROCHEMISTRY
卷 113, 期 6, 页码 1589-1597出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1471-4159.2010.06717.x
关键词
branching; migration; myelin; oligodendroglia; oligodendrocyte precursor cell; RhoGTPase
资金
- Multiple Sclerosis Society of Canada
- Scottish Rite Charitable Foundation (SRCF) 3
- CIHR Frederick Banting
- Charles Best Canada Graduate Scholarship
- FRSQ Chercheur National Award
- Killam Foundation
P>The guidance cue netrin-1 and its receptor Deleted in Colorectal Cancer play distinct roles during different stages of oligodendrocyte development. A gradient of netrin-1 repels migrating oligodendrocyte precursor cells (OPCs) in the embryonic spinal cord by promoting process collapse, but later in development netrin-1 increases oligodendrocyte process extension and branching. Here we investigate the intracellular mechanism that governs this switch in response to netrin-1, and focus on the role of the GTPase RhoA and its effector Rho Kinase (ROCK) downstream of netrin-1 in OPCs and maturing oligodendrocytes. In OPCs, we show that netrin-1 induces a sustained increase in RhoA activity that requires Deleted in Colorectal Cancer function. Furthermore, we demonstrate that activation of RhoA and ROCK is required for the reduction in OPC process length triggered by netrin-1, and for the chemorepellent response made by OPCs to netrin-1. Unlike OPCs, application of netrin-1 to oligodendrocytes decreases RhoA activity. We demonstrate that inactivation of RhoA is essential for netrin-1 to increase oligodendrocyte process branching. We conclude that netrin-1 induces distinct morphological responses in OPCs and oligodendrocytes through differential regulation of RhoA activity.
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