期刊
JOURNAL OF NEUROCHEMISTRY
卷 79, 期 2, 页码 417-425出版社
BLACKWELL SCIENCE LTD
DOI: 10.1046/j.1471-4159.2001.00559.x
关键词
allopregnanolone; amygdala; neuroactive steroids; progesterone; transgenic mice; Y-1 receptor for neuropeptide Y
资金
- Telethon [D.082] Funding Source: Medline
The neurosteroid allopregnanolone, a reduced metabolite of progesterone, induces anxiolytic effects by enhancing GABAA receptor function. Neuropeptide Y (NPY) and GABA are thought to interact functionally in the amygdala, and this interaction may be important in the regulation of anxiety. By using Y1R/LacZ transgenic mice, which harbour a fusion construct comprising the promoter of the mouse gene for the Y-1 receptor for NPY linked to the lacZ gene, we previously showed that long-term treatment with benzodiazepine receptor ligands modulates Y-1 receptor gene expression in the medial amygdala. We have now investigated the effects of prolonged treatment with progesterone or allopregnanolone on Y1R/LacZ transgene expression, as determined by quantitative histochemical analysis of beta -galactosiclase activity. Progesterone increased both the cerebrocortical concentration of allopregnanolone and p-galactosidase expression in the medial amygdala. Finasteride, a 5 alpha -reductase inhibitor, prevented both of these effects. Long-term administration of allopregnanolone also increased both the cortical concentration of this neurosteroid and transgene expression in the medial amygdala. Treatment with neither progesterone nor allopregnanolone affected P-galactosidase activity in the medial habenula. These data suggest that allopregnanolone regulates Y-1 receptor gene expression through modulation of GABA(A) receptor function, and they provide further support for a functional interaction between GABA and neuropeptide Y in the amygdala.
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