期刊
JOURNAL OF NEURO-ONCOLOGY
卷 111, 期 1, 页码 71-81出版社
SPRINGER
DOI: 10.1007/s11060-012-0992-3
关键词
Glioma; miRNA; miR-21; LNA; In situ hybridization; Prognosis
资金
- Region of Southern Denmark
- Research Council for Health and Disease (FSS)
- Else and Age Gronbaek-Olsen
- Foundation of Merchant M. Kristian Kjaer
- wife Margrethe Kjaer born la Cour-Holmen
- fmr. Dir. Leo Nielsen and wife Karen Margrethe Nielsen for medical basic research
- Beckett Foundation
- Research Foundation of University of Southern Denmark
- Foundation of Fam. Hede Nielsen
- Foundation of Margot and John Friberg
- Foundation of Dir. Jacob Madsen and wife Olga Madsen
- Carl and Ellen Hertz for Danish medicine and science
- Foundation of engineer Bent Bogh and wife Inge Bogh
High-grade gliomas are some of the most lethal forms of human cancer, and new clinical biomarkers and therapeutic targets are highly needed. MicroRNAs (miRNAs), a group of short noncoding RNAs, hold great potential as new biomarkers and targets as they are commonly deregulated in a variety of diseases including gliomas. MicroRNA-21 (miR-21) is the most consistently overexpressed miRNA in several cancers including gliomas and is therefore very promising as a useful clinical biomarker and therapeutic target. To better understand the role of miR-21 in gliomas, paraffin-embedded glioma tissue samples from 193 patients with grade I, II, III, and IV tumors were analyzed by in situ hybridization (ISH) using LNA-DNA chimeric probes. We found miR-21 expression in tumor cells and tumor-associated blood vessels, whereas no expression was seen in adjacent normal brain parenchyma. Using advanced image analysis we obtained quantitative estimates reflecting the miR-21 expression levels in each of these compartments. The miR-21 levels correlated significantly with grade [p = 0.027, r (s) = 0.161, 95 % confidence interval (CI), 0.015-0.301] with the highest levels measured in glioblastomas. Only tumor cell miR-21 was associated with poor prognosis when adjusting for known clinical parameters (age, grade, and sex) in a multivariate analysis [p = 0.049, hazard ratio (HR) = 1.545, 95 % CI, 1.002-2.381]. In conclusion, we have shown that miR-21 is located in both tumor cells and tumor blood vessels and that its level in the tumor cell compartment holds unfavorable prognostic value in gliomas.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据